Development of reagents and assays for the detection of pathogenic Burkholderia species

Faraday Discuss. 2011;149:23-36; discussion 63-77. doi: 10.1039/c005422b.


Rapid detection of the category B biothreat agents Burkholderia pseudomallei and Burkholderia mallei in acute infections is critical to ensure that appropriate treatment is administered quickly to reduce an otherwise high probability of mortality (ca. 40% for B. pseudomallei). We are developing assays that can be used in clinical laboratories or security applications for the direct detection of surface-localized and secreted macromolecules produced by these organisms. We present our current medium-throughout approach for target selection and production of Burkholderia macromolecules and describe the generation of a Fab molecule targeted to the B. mallei BimA protein. We also present development of prototype assays for detecting Burkholderia species using anti-lipopolysaccharide antibodies.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Burkholderia mallei / isolation & purification*
  • Burkholderia mallei / metabolism
  • Burkholderia pseudomallei / isolation & purification*
  • Burkholderia pseudomallei / metabolism
  • Chaperonin 60 / chemistry
  • Chaperonin 60 / metabolism
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Glanders / diagnosis
  • Glanders / microbiology*
  • Humans
  • Melioidosis / diagnosis
  • Melioidosis / microbiology*
  • Microfilament Proteins / chemistry
  • Microfilament Proteins / metabolism


  • BimA protein, Burkholderia pseudomallei
  • Chaperonin 60
  • Microfilament Proteins