Human mesenchymal stem cell-conditioned medium improves cardiac function following myocardial infarction

Stem Cell Res. 2011 May;6(3):206-14. doi: 10.1016/j.scr.2011.01.001. Epub 2011 Jan 28.


Recent studies suggest that the therapeutic effects of stem cell transplantation following myocardial infarction (MI) are mediated by paracrine factors. One of the main goals in the treatment of ischemic heart disease is to stimulate vascular repair mechanisms. Here, we sought to explore the therapeutic angiogenic potential of mesenchymal stem cell (MSC) secretions. Human MSC secretions were collected as conditioned medium (MSC-CM) using a clinically compliant protocol. Based on proteomic and pathway analysis of MSC-CM, an in vitro assay of HUVEC spheroids was performed identifying the angiogenic properties of MSC-CM. Subsequently, pigs were subjected to surgical left circumflex coronary artery ligation and randomized to intravenous MSC-CM treatment or non-CM (NCM) treatment for 7 days. Three weeks after MI, myocardial capillary density was higher in pigs treated with MSC-CM (645 ± 114 vs 981 ± 55 capillaries/mm(2); P = 0.021), which was accompanied by reduced myocardial infarct size and preserved systolic and diastolic performance. Intravenous MSC-CM treatment after myocardial infarction increases capillary density and preserves cardiac function, probably by increasing myocardial perfusion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiogenesis Inducing Agents / metabolism*
  • Angiogenesis Inducing Agents / therapeutic use*
  • Animals
  • Blood Pressure / drug effects
  • Coronary Vessels / drug effects
  • Coronary Vessels / physiopathology
  • Coronary Vessels / surgery
  • Culture Media, Conditioned / metabolism*
  • Disease Models, Animal
  • Heart / physiopathology*
  • Humans
  • Mesenchymal Stem Cells / metabolism*
  • Myocardial Infarction / drug therapy*
  • Myocardial Infarction / physiopathology
  • Myocardial Infarction / surgery
  • Neovascularization, Physiologic / drug effects
  • Random Allocation
  • Swine


  • Angiogenesis Inducing Agents
  • Culture Media, Conditioned