Isolation of methanobactin from the spent media of methane-oxidizing bacteria

Methods Enzymol. 2011;495:259-69. doi: 10.1016/B978-0-12-386905-0.00017-6.

Abstract

Chalkophores are low molecular mass modified peptides involved in copper acquisition in methane-oxidizing bacteria (MOB). A screening method for the detection of this copper-binding molecule is presented in Chapter 16. Here we describe methods to (1) maximize expression and secretion of chalkophores, (2) concentrate chalkophores from the spent media of MOB, and (3) purify chalkophores.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Imidazoles / isolation & purification*
  • Methylobacterium / chemistry*
  • Methylococcus capsulatus / chemistry*
  • Methylocystaceae / chemistry*
  • Methylosinus trichosporium / chemistry*
  • Oligopeptides / isolation & purification*

Substances

  • Imidazoles
  • Oligopeptides
  • methanobactin