Nipkow confocal imaging from deep brain tissues

J Integr Neurosci. 2011 Mar;10(1):121-9. doi: 10.1142/S0219635211002658.


One of the problems in imaging from brain tissues is light-scattering. Thus, multiphoton laser scanning microscopy is widely used to optically access fluorescent signals located deeply in tissues. Here we report that Nipkow-type spinning-disk one-photon confocal microscopy, which embodies high temporal resolution and slow photobleaching, is also capable of imaging tissues to a depth of up to 150 μm. Using a Nipkow-disk microscope, we conducted functional multi-cell calcium imaging of CA3 neurons from in toto intact hippocampal preparations and astrocytes from in vivo neocortical layer 1. This novel application of Nipkow-disk microscopy expands the potential usefulness of this type of microscopy and will contribute to our understanding of natural neuronal microcircuitry.

Publication types

  • Comparative Study

MeSH terms

  • Aniline Compounds / analysis
  • Animals
  • Astrocytes / chemistry
  • Astrocytes / cytology
  • Brain / cytology
  • CA3 Region, Hippocampal / chemistry
  • CA3 Region, Hippocampal / cytology*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred ICR
  • Mice, Transgenic
  • Microscopy, Confocal / instrumentation
  • Microscopy, Confocal / methods
  • Neocortex / chemistry
  • Neocortex / cytology*
  • Photons*
  • Xanthenes / analysis


  • Aniline Compounds
  • Fluo 4
  • Xanthenes