Isolation and culture of human multipotent stromal cells from the pancreas

Methods Mol Biol. 2011;698:123-40. doi: 10.1007/978-1-60761-999-4_10.

Abstract

Mesenchymal stem cells, also termed multipotent mesenchymal stromal cells (MSCs), can be isolated from most adult tissues. Although the exact origin of MSCs expanded from the human pancreas has not been resolved, we have developed protocols to isolate and expand MSCs from human pancreatic tissue that remains after islet procurement. Similar to techniques used to isolate MSCs from bone marrow, pancreatic MSCs are isolated based on their cell adherence, expression of several cell surface antigens, and multilineage differentiation. The protocols for isolating, characterizing, and differentiating MSCs from the pancreas are presented in this chapter.

MeSH terms

  • Adipogenesis
  • Alkaline Phosphatase / metabolism
  • Cell Aggregation
  • Cell Culture Techniques / methods*
  • Cell Separation / methods*
  • Cryopreservation
  • Flow Cytometry
  • Humans
  • Lipid Metabolism
  • Multipotent Stem Cells / cytology*
  • Osteogenesis
  • Pancreas / cytology*
  • Staining and Labeling

Substances

  • Alkaline Phosphatase