Structure and biosynthesis of the xylose-containing carbohydrate moiety of rice alpha-amylase

Eur J Biochem. 1990 Jul 31;191(2):287-95. doi: 10.1111/j.1432-1033.1990.tb19122.x.


Suspension-cultured cells of rice secrete alpha-amylase into the culture medium. It has been shown that the mature form of the alpha-amylase contains xylose-bearing N-linked oligosaccharide: (formula; see text) We demonstrate that suspension-cultured cells of rice secrete alpha-amylase containing oligomannose-type oligosaccharides in the presence of 1-deoxymannojirimycin or tris(hydroxymethyl)aminomethane. On the other hand, alpha-amylase purified from germinated rice seedlings contains several kinds of oligomannose-type and N-acetyllactosamine-type oligosaccharides. The processing pathway of oligosaccharide moieties in rice cells is discussed on the basis of a comparison of these oligosaccharides structures.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1-Deoxynojirimycin
  • Carbohydrate Sequence
  • Cells, Cultured
  • Chromatography, High Pressure Liquid
  • Glucosamine / analogs & derivatives
  • Glucosamine / pharmacology
  • Magnetic Resonance Spectroscopy
  • Molecular Sequence Data
  • Oligosaccharides / analysis
  • Oligosaccharides / biosynthesis*
  • Oryza / enzymology*
  • Temperature
  • Tunicamycin / pharmacology
  • Xylose / analysis*
  • alpha-Amylases / analysis
  • alpha-Amylases / biosynthesis*


  • Oligosaccharides
  • Tunicamycin
  • 1-Deoxynojirimycin
  • Xylose
  • alpha-Amylases
  • Glucosamine