Molecular epidemiology and clinical presentation of human adenovirus infections in Kansas City children

J Clin Virol. 2011 Jun;51(2):126-31. doi: 10.1016/j.jcv.2011.02.014. Epub 2011 Mar 26.


Background: A significant increase in adenovirus detection among patients at the Children's Mercy Hospital, Kansas City was observed between June 2007 and January 2008.

Objective: To molecularly characterize the human adenoviruses and describe their association with clinical illness in children.

Study design: One hundred adenovirus-positive specimens from 79 children were typed by hexon gene sequence typing method. Restriction enzyme analysis (REA) was performed on isolates of HAdV-3, -7 and -14 to identify genomic variants. Medical records were reviewed to understand the clinical illnesses associated with adenovirus serotypes and genome types.

Results: The most prevalent HAdV serotypes were HAdV-3 (37%), HAdV-7 (25%), HAdV-1 (16%), HAdV-2 (8%). HAdV infection was common in children ≤3 years of age (71%) versus children >3 years (29%). Majority of the HAdV-infected children were hospitalized (78%); 22/79 (28%) stayed >3 days and 8/79 (10%) required intensive care unit stay. Hospitalization rates for HAdV-3 (36%) and HAdV-7 (25%) were comparable. REA data indicated that HAdV-3a2 was the predominant HAdV-3 genome type. Two novel genomic variants of HAdV-3 exhibiting unique BglII or BstEII profiles were identified in isolates from patients with bronchiolitis. All HAdV-7 and -14 isolates were identified as corresponding to genome types 7d2 and 14p1, respectively.

Conclusions: In Kansas City, we noticed an increase in the incidence of HAdV-7 (25%; n=24/98) infections compared to the previous two years (6%; n=6/107). Two new genomic variants of HAdV-3 appear to have emerged in our area and seem to be associated with lower respiratory tract infections in children.

MeSH terms

  • Adenovirus Infections, Human / epidemiology*
  • Adenovirus Infections, Human / pathology*
  • Adenovirus Infections, Human / virology
  • Adenoviruses, Human / classification*
  • Adenoviruses, Human / genetics*
  • Adenoviruses, Human / isolation & purification
  • Child
  • Child, Preschool
  • Cluster Analysis
  • DNA, Viral / genetics*
  • Female
  • Genotype
  • Humans
  • Kansas / epidemiology
  • Male
  • Molecular Epidemiology
  • Prohibitins
  • Restriction Mapping
  • Sequence Analysis, DNA
  • Serotyping


  • DNA, Viral
  • PHB2 protein, human
  • Prohibitins