Structural feature of bent DNA recognized by HMGB1

J Am Chem Soc. 2011 Apr 20;133(15):5788-90. doi: 10.1021/ja2013399. Epub 2011 Mar 28.

Abstract

High Mobility Group Box 1 (HMGB1) protein, a potential therapeutic target, binds bent DNAs structure-specifically. Here we report on a crucial structural feature of the bent DNA required for strong binding to HMGB1. NMR structures of two bent DNA oligomers, only one of which binds strongly to HMGB1, revealed that the presence of a pocket structure on the minor groove is crucial for strong binding through penetration of a phenylalanine residue.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / chemistry
  • DNA / metabolism*
  • HMGB1 Protein / metabolism*
  • Humans
  • Models, Molecular
  • Nuclear Magnetic Resonance, Biomolecular
  • Nucleic Acid Conformation
  • Protein Binding

Substances

  • HMGB1 Protein
  • DNA