Inhibition of UV-induced ROS and collagen damage by Phyllanthus emblica extract in normal human dermal fibroblasts

J Cosmet Sci. Jan-Feb 2011;62(1):49-56.

Abstract

As a part of ongoing research for novel natural cosmeceutical actives from plant extracts, this study demonstrates that Phyllanthus emblica fruit extract has shown its efficacy in protection against ultraviolet-B (UVB) irradiation-induced reactive oxygen species (ROS) and collagen damage in normal human dermal fibroblasts. At a concentration of 0.5 mg/ml, emblica extract showed a significant response of 9.5 ± 0.28-fold protection from UVB induced-collagen damage as compared to untreated cells. A known active, ascorbic acid, at a concentration of 0.5 mg/ml, showed 3.7 ± 0.07-fold protection from UVB-induced collagen damage. While the untreated cells showed 84 ± 1.4% induction in ROS on UVB irradiation as compared to the non-irradiated cells, emblica extract treatment inhibited the induction of ROS to 15 ± 4% at a concentration of 0.5 mg/ml. Ascorbic acid inhibited the induction in ROS to 64 ± 2% at a concentration of 0.5 mg/ml. Emblica extract is a significantly better natural active, with promising cosmeceutical benefits against photoaging.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Collagen / metabolism*
  • Dermis / cytology
  • Fibroblasts* / drug effects
  • Fibroblasts* / radiation effects
  • Humans
  • Phyllanthus emblica / chemistry*
  • Plant Extracts / chemistry*
  • Plant Extracts / pharmacology*
  • Reactive Oxygen Species / metabolism*
  • Ultraviolet Rays

Substances

  • Plant Extracts
  • Reactive Oxygen Species
  • Collagen