Sensitive (13)C- (13)C correlation spectra of amyloid fibrils at very high spinning frequencies and magnetic fields

J Biomol NMR. 2011 Jun;50(2):129-36. doi: 10.1007/s10858-011-9501-9. Epub 2011 Mar 29.

Abstract

Sensitive 2D solid-state (13)C-(13)C correlation spectra of amyloid β fibrils have been recorded at very fast spinning frequencies and very high magnetic fields. It is demonstrated that PARIS-xy recoupling using moderate rf amplitudes can provide structural information by promoting efficient magnetization transfer even under such challenging experimental conditions. Furthermore, it has been shown both experimentally and by numerical simulations that the method is not very sensitive to dipolar truncation effects and can reveal direct transfer across distances of about 3.5-4 Å.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amyloid / chemistry*
  • Amyloid beta-Peptides / chemical synthesis
  • Amyloid beta-Peptides / chemistry
  • Carbon Isotopes / chemistry*
  • Computer Simulation
  • Models, Molecular
  • Nuclear Magnetic Resonance, Biomolecular*

Substances

  • Amyloid
  • Amyloid beta-Peptides
  • Carbon Isotopes