Role of the probiotic strain Lactobacillus paracasei LMGP22043 carried by artichokes in influencing faecal bacteria and biochemical parameters in human subjects

J Appl Microbiol. 2011 Jul;111(1):155-64. doi: 10.1111/j.1365-2672.2011.05019.x. Epub 2011 Apr 20.


Aims: To evaluate the positive influence of the probiotic strain Lactobacillus paracasei LMGP22043 carried by artichokes into the human gut with special reference to faecal bacterial balance, short-chain fatty acid concentrations and enzyme activities in a randomized, double-blind human trial in comparison with probiotic-free artichokes (control).

Methods: Twenty subjects were randomized into two groups, which consumed daily 180 g of the artichoke product (probiotic or control) during two 15-day study periods (periods 1 and 2) separated by a 15-day washout in a crossover manner. Faecal samples were subjected to microbiological and biochemical analyses, and a strain-specific PCR was performed to monitor the probiotic strain.

Results: The probiotic strain, transported by the vegetable matrix, transiently colonized the gut of 17/20 subjects (median 6·87 log CFU g⁻¹ faeces), antagonized Escherichia coli and Clostridium spp. and increased the genetic diversity of lactic population based on REP-PCR profiles, mainly after period 1.

Conclusions: The probiotic L. paracasei LMGP22043 successfully colonized the human gut and positively influenced faecal bacteria and biochemical parameters.

Significance and impact of the study: The association of the probiotic L. paracasei with a food carrier rich in fibre can represent a new strategy for favouring a daily supply of probiotics and attracting more consumers to vegetable food fortified with probiotic strains.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Cynara scolymus / microbiology*
  • Diet*
  • Double-Blind Method
  • Fatty Acids, Volatile / analysis
  • Feces / chemistry
  • Feces / enzymology
  • Feces / microbiology
  • Female
  • Gastrointestinal Tract / microbiology*
  • Humans
  • Lactobacillus*
  • Male
  • Middle Aged
  • Probiotics / administration & dosage*
  • Probiotics / analysis


  • Fatty Acids, Volatile