Schizosaccharomyces pombe Arc3 is a conserved subunit of the Arp2/3 complex required for polarity, actin organization, and endocytosis

Yeast. 2011 Jun;28(6):495-503. doi: 10.1002/yea.1853. Epub 2011 Mar 30.


We characterized the Schizosaccharomyces pombe arc3 gene, whose product shares sequence homology with that of the budding yeast ARC18 and human ARPC3/p21 subunits of the Arp2/3 complex. Our data showed that Arc3p co-localizes with F-actin patches at the cell ends, but not with F-actin cables or the equatorial actin ring, and binds other subunits of the Arp2/3 complex. Gene deletion analysis showed that arc3 is essential for viability. When arc3 expression was repressed, F-actin patches became dispersed throughout the cell with greatly reduced mobility. Furthermore, in arc3-repressed cells, endocytosis was also inhibited. Human ARPC3 rescued the viability of the Sz. pombe arc3 null mutant; in addition, ARPC3 also localized to F-actin patches in human cells. These data suggest that Arc3p is an evolutionarily conserved subunit of the Arp2/3 complex required for proper F-actin organization and efficient endocytosis.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actin-Related Protein 2-3 Complex / chemistry*
  • Actin-Related Protein 2-3 Complex / metabolism*
  • Actins / metabolism*
  • Endocytosis*
  • Fungal Proteins / isolation & purification*
  • Fungal Proteins / metabolism*
  • Gene Deletion
  • Genes, Essential
  • Genes, Fungal
  • Genetic Complementation Test
  • Microbial Viability
  • Schizosaccharomyces / metabolism
  • Schizosaccharomyces / physiology*


  • Actin-Related Protein 2-3 Complex
  • Actins
  • Fungal Proteins