Th17/Treg Imbalance in Adult Patients With Minimal Change Nephrotic Syndrome

Clin Immunol. 2011 Jun;139(3):314-20. doi: 10.1016/j.clim.2011.02.018. Epub 2011 Mar 1.

Abstract

To determine whether Th17/Treg balance was abnormal in adult patients with minimal change nephrotic syndrome (MCNS), we studied 25 patients with new-onset MCNS and 20 normal persons. The results showed that MCNS patients exhibited a significant increase in Th17 number, Th17-related cytokines (IL-17 and IL-23), and transcription factor (RORγt) levels, as well as an obvious decrease in Treg number, Treg-related cytokines (TGF-β1 and IL-10), and transcription factor (Foxp3) levels. The Th17/Treg ratios increased along with increased proteinuria and decreased albumin levels in patients with MCNS. IL-17 protein expression was also detected in the renal biopsy tissue of MCNS patients, particularly in patients with acute renal failure. Further, Th17/Treg balance returned to normal after effective corticosteroids therapy in 16 MCNS patients. These results indicated that Th17/Treg imbalance existed in MCNS patients, suggesting a potential role of Th17/Treg imbalance in the pathogenesis of MCNS.

MeSH terms

  • Adult
  • Biopsy
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Flow Cytometry
  • Forkhead Transcription Factors / blood
  • Forkhead Transcription Factors / genetics
  • Forkhead Transcription Factors / immunology
  • Humans
  • Immunohistochemistry
  • Interleukin-10 / blood
  • Interleukin-10 / immunology
  • Interleukin-17 / blood
  • Interleukin-17 / immunology
  • Interleukin-23 / blood
  • Interleukin-23 / immunology
  • Male
  • Middle Aged
  • Nephrosis, Lipoid / blood
  • Nephrosis, Lipoid / immunology
  • Nephrosis, Lipoid / pathology
  • Nuclear Receptor Subfamily 1, Group F, Member 3 / blood
  • Nuclear Receptor Subfamily 1, Group F, Member 3 / genetics
  • Nuclear Receptor Subfamily 1, Group F, Member 3 / immunology
  • RNA / chemistry
  • RNA / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • T-Lymphocytes, Regulatory / immunology*
  • T-Lymphocytes, Regulatory / pathology
  • Th17 Cells / immunology*
  • Th17 Cells / pathology
  • Transforming Growth Factor beta1 / blood
  • Transforming Growth Factor beta1 / immunology

Substances

  • FOXP3 protein, human
  • Forkhead Transcription Factors
  • Interleukin-17
  • Interleukin-23
  • Nuclear Receptor Subfamily 1, Group F, Member 3
  • Transforming Growth Factor beta1
  • Interleukin-10
  • RNA