The nematode spliced leader RNA participates in trans-splicing as an Sm snRNP

EMBO J. 1990 Nov;9(11):3667-73. doi: 10.1002/j.1460-2075.1990.tb07578.x.

Abstract

The trans-spliced leader RNA (SL RNA) of nematodes resembles U snRNAs both in cap structure and in the presence of a consensus Sm binding site. We show here that synthetic SL RNA, synthesized by in vitro transcription, is efficiently used as a spliced leader donor in trans-splicing reactions catalyzed by a cell free extract prepared from developing embryos of the parasitic nematode, Ascaris lumbricoides. Efficient utilization of synthetic SL RNA requires a functional Sm binding site. Mutations within the Sm binding sequence that prevent immunoprecipitation by Sm antisera and prevent cap trimethylation abolish trans-splicing. The effect on trans-splicing is not due to undermethylation of the cap structure.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Ascaris / genetics*
  • Autoantigens / physiology
  • Base Sequence
  • Binding Sites
  • DNA Mutational Analysis
  • In Vitro Techniques
  • Molecular Sequence Data
  • Nucleic Acid Precursors / metabolism
  • Oligonucleotides
  • RNA Caps
  • RNA Splicing*
  • RNA, Messenger / metabolism
  • Ribonucleoproteins / physiology*
  • Ribonucleoproteins, Small Nuclear
  • snRNP Core Proteins

Substances

  • Autoantigens
  • Nucleic Acid Precursors
  • Oligonucleotides
  • RNA Caps
  • RNA, Messenger
  • Ribonucleoproteins
  • Ribonucleoproteins, Small Nuclear
  • snRNP Core Proteins