Hepatoprotective effects of chestnut (Castanea crenata) inner shell extract against chronic ethanol-induced oxidative stress in C57BL/6 mice

Food Chem Toxicol. 2011 Jul;49(7):1537-43. doi: 10.1016/j.fct.2011.03.045. Epub 2011 Mar 30.


This study was carried out to evaluate the protective effects of chestnut inner shell extract (CISE) on chronic ethanol-induced oxidative stress in liver. Mice were fed a control liquid diet (Normal-control), liquid diet containing ethanol alone (EtOH+Vehicle), or were administered CISE and ethanol (EtOH+CISE) for 6 weeks. Administration of ethanol induced liver damage with significant increase of plasma GOT, GPT, hepatic triglyceride (TG) and thiobarbituric acid reactive substance (TBARS) levels. By contrast, co-treatment of CISE with ethanol significantly decreased the activities of GOT and GPT in the plasma, and hepatic TG and TBARS levels. Histological observations were consistent with the result obtained from hepatic lipid quantification. Moreover, CISE treatment with ethanol decreased CYP2E1 expression and increased activities of catalase and superoxide dismutase, which were significantly inhibited by treatment with ethanol alone. To determine the active compound of CISE, fractionation of CISE was conducted and scoparone and scopoletin were identified as main compounds. These compounds were also shown to inhibit the ethanol-induced reduction in antioxidant enzyme activity in an in vitro model system. These results suggest that CISE has protective effects against ethanol-induced oxidative damage, possibly by inhibition of lipid accumulation, peroxidation and increase of antioxidant defense system in the liver.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Catalase / genetics
  • Catalase / metabolism
  • Chemical and Drug Induced Liver Injury, Chronic / drug therapy*
  • Coumarins / isolation & purification
  • Coumarins / pharmacology
  • Cytochrome P-450 CYP2E1 / genetics
  • Cytochrome P-450 CYP2E1 Inhibitors
  • Ethanol / toxicity*
  • Fagaceae / chemistry*
  • Hep G2 Cells
  • Humans
  • Lipid Peroxidation / drug effects
  • Liver / drug effects
  • Liver / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Oxidative Stress / drug effects*
  • Plant Extracts / pharmacology*
  • Scopoletin / isolation & purification
  • Scopoletin / pharmacology
  • Superoxide Dismutase / genetics
  • Superoxide Dismutase / metabolism
  • Thiobarbituric Acid Reactive Substances / analysis
  • Triglycerides / analysis


  • Coumarins
  • Cytochrome P-450 CYP2E1 Inhibitors
  • Plant Extracts
  • Thiobarbituric Acid Reactive Substances
  • Triglycerides
  • Ethanol
  • Catalase
  • Cytochrome P-450 CYP2E1
  • Superoxide Dismutase
  • scoparone
  • Scopoletin