Isolation and expression of cDNA for different forms of hepatocyte growth factor from human leukocyte

Biochem Biophys Res Commun. 1990 Oct 15;172(1):321-7. doi: 10.1016/s0006-291x(05)80212-8.

Abstract

Human leukocyte cDNA library was screened to isolate cDNA clones coding for hepatocyte growth factor using cDNA from human liver as a probe. Nucleotide and deduced amino acid sequences were analyzed for two of four clones obtained. One of them contained an open reading frame coding for a polypeptide chain of 728 amino acid residues like that of cDNA clone derived from human liver. In another clone a spontaneous deletion of 15 base pairs was found within the coding sequence. When expressed transiently using COS-1 cells both clones produced protein with similar biological activity against rat hepatocyte in vitro.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Division
  • Cell Line
  • Cloning, Molecular
  • DNA / blood
  • DNA / genetics*
  • DNA / isolation & purification
  • Gene Library
  • Growth Substances / blood
  • Growth Substances / genetics*
  • Hepatocyte Growth Factor
  • Humans
  • Leukocytes / metabolism*
  • Liver / metabolism
  • Molecular Sequence Data
  • Oligonucleotide Probes
  • Restriction Mapping
  • Transfection

Substances

  • Growth Substances
  • Oligonucleotide Probes
  • Hepatocyte Growth Factor
  • DNA

Associated data

  • GENBANK/M60718