A more efficient method to generate integration-free human iPS cells

Nat Methods. 2011 May;8(5):409-12. doi: 10.1038/nmeth.1591. Epub 2011 Apr 3.


We report a simple method, using p53 suppression and nontransforming L-Myc, to generate human induced pluripotent stem cells (iPSCs) with episomal plasmid vectors. We generated human iPSCs from multiple donors, including two putative human leukocyte antigen (HLA)-homozygous donors who match ∼20% of the Japanese population at major HLA loci; most iPSCs are integrated transgene-free. This method may provide iPSCs suitable for autologous and allologous stem-cell therapy in the future.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Asians / genetics
  • Cell Culture Techniques / methods*
  • Electroporation
  • Gene Expression Profiling
  • Gene Frequency
  • Genetic Vectors
  • HLA Antigens / genetics
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / immunology
  • Induced Pluripotent Stem Cells / metabolism
  • Plasmids / genetics
  • Tissue Donors


  • HLA Antigens