A genome-scale shRNA resource for transgenic RNAi in Drosophila

Nat Methods. 2011 May;8(5):405-7. doi: 10.1038/nmeth.1592. Epub 2011 Apr 3.


Existing transgenic RNAi resources in Drosophila melanogaster based on long double-stranded hairpin RNAs are powerful tools for functional studies, but they are ineffective in gene knockdown during oogenesis, an important model system for the study of many biological questions. We show that shRNAs, modeled on an endogenous microRNA, are extremely effective at silencing gene expression during oogenesis. We also describe our progress toward building a genome-wide shRNA resource.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • DNA Primers / genetics
  • Drosophila melanogaster / genetics*
  • Female
  • Gene Knockdown Techniques
  • Genetic Techniques
  • Genetic Vectors
  • Genome, Insect*
  • MicroRNAs / genetics
  • Oogenesis / genetics
  • RNA Interference*
  • RNA, Small Interfering / genetics


  • DNA Primers
  • MicroRNAs
  • RNA, Small Interfering

Associated data

  • GEO/GSE27039