Macrophage colony stimulating factor restores in vivo bone resorption in the op/op osteopetrotic mouse

Endocrinology. 1990 Nov;127(5):2592-4. doi: 10.1210/endo-127-5-2592.


The op/op variant of murine osteopetrosis is a recessive mutation characterized by impaired bone resorption due to lack of osteoclasts. Cultured osteoblasts and fibroblasts from this mutant do not secrete M-CSF activity and resident macrophages are absent in bone marrow. This failure has been related to a mutation within the M-CSF coding region. We report now that the administration of recombinant human M-CSF (rhM-CSF) corrects in vivo the impaired bone resorption in this animal. The treatment restores the bone marrow cavity virtually absent in the op/op animal and induces the appearance of resorbing osteoclasts and of resident bone marrow macrophages. This proves that the deficiency of M-CSF is the cause of the op/op bone disorder and that this cytokine is directly or indirectly necessary for physiological osteoclastogenesis, the resulting bone resorption and for the establishment of bone marrow hemopoiesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Resorption
  • Cell Count
  • Dose-Response Relationship, Drug
  • Female
  • Macrophage Colony-Stimulating Factor / pharmacology*
  • Macrophages / pathology
  • Male
  • Metacarpus / pathology
  • Metacarpus / physiopathology
  • Mice
  • Mice, Mutant Strains
  • Monocytes / pathology
  • Osteopetrosis / genetics
  • Osteopetrosis / pathology
  • Osteopetrosis / physiopathology*
  • Receptor, Macrophage Colony-Stimulating Factor
  • Time Factors


  • Macrophage Colony-Stimulating Factor
  • Receptor, Macrophage Colony-Stimulating Factor