Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H(+)-adenosine triphosphatase

P M Kane; C T Yamashiro; D F Wolczyk; N Neff; M Goebl; T H Stevens

doi:10.1126/science.2146742

Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H(+)-adenosine triphosphatase

Science. 1990 Nov 2;250(4981):651-7. doi: 10.1126/science.2146742.

Authors

P M Kane¹, C T Yamashiro, D F Wolczyk, N Neff, M Goebl, T H Stevens

Affiliation

¹ Institute of Molecular Biology, University of Oregon, Eugene 97403.

PMID: 2146742
DOI: 10.1126/science.2146742

Abstract

The TFP1 gene of the yeast Saccharomyces cerevisiae encodes two proteins: the 69-kilodalton (kD) catalytic subunit of the vacuolar proton-translocating adenosine triphosphatase (H(+)-ATPase) and a 50-kD protein. The 69-kD subunit is encoded by the 5' and 3' thirds of the TFP1 coding region, whereas the 50-kD protein is encoded by the central third. Evidence is presented that both the 69-kD and 50-kD proteins are obtained from a single translation product that is cleaved to release the 50-kD protein and spliced to form the 69-kD subunit.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Animals
Genes, Fungal*
Protein Biosynthesis
Protein Processing, Post-Translational*
Proton-Translocating ATPases / biosynthesis*
Proton-Translocating ATPases / genetics
RNA, Messenger / analysis
Rabbits
Saccharomyces cerevisiae / enzymology*
Vacuoles / enzymology*

Substances

RNA, Messenger
Proton-Translocating ATPases

Grants and funding

GM38006/GM/NIGMS NIH HHS/United States