Genomic characterization of ten novel cutaneous human papillomaviruses from keratotic lesions of immunosuppressed patients

J Gen Virol. 2011 Jul;92(Pt 7):1585-1594. doi: 10.1099/vir.0.030593-0. Epub 2011 Apr 6.


Viral warts from immunosuppressed organ transplant recipients (OTR) persist over years and may progress into non-melanoma skin cancer. The types of human papillomaviruses (HPV) in such lesions are different from that seen in the general population. A subset of these lesions is not infected with the classical wart-associated HPV types. In order to gain a better understanding of the HPV types in those lesions, we isolated ten novel HPVs from persisting keratotic lesions of immunosuppressed OTRs by rolling circle amplification and subsequent long-template PCR. Additionally, we sequenced and characterized the whole genome of the ten novel HPV types. Phylogenetic analyses revealed that nine HPV types belonged to the genus Gammapapillomavirus (γ-PV) and one to the genus Betapapillomavirus. In a phylogenetic analysis using L1 fragments of human and non-human PV types, primate papillomaviruses and our novel HPV types nested within the genus γ-PV in a highly polyphyletic pattern. This study significantly broadens the knowledge concerning the diversity and evolution of the poorly known γ-PV types.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Female
  • Genome, Viral
  • Genomics
  • Humans
  • Immunocompromised Host
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Organ Transplantation / adverse effects
  • Papillomaviridae / classification
  • Papillomaviridae / genetics*
  • Papillomaviridae / isolation & purification*
  • Papillomavirus Infections / etiology
  • Papillomavirus Infections / virology*
  • Phylogeny
  • Skin Diseases / immunology
  • Skin Diseases / virology*

Associated data

  • GENBANK/GQ246951
  • GENBANK/GU117629
  • GENBANK/GU117630
  • GENBANK/GU117631
  • GENBANK/GU117632
  • GENBANK/GU117633
  • GENBANK/GU117634
  • GENBANK/GU129016
  • GENBANK/GU225708
  • GENBANK/GU233853