Total polyphenolic (flavonoids) content and antioxidant capacity of different Ziziphora clinopodioides Lam. extracts

Pharmacogn Mag. 2011 Jan;7(25):65-8. doi: 10.4103/0973-1296.75904.

Abstract

Objective: This paper investigates the total polyphenolic and flavonoid content as well as the antioxidant activity of Ziziphora clinopodioides Lam. extracts of different polarity.

Materials and methods: The total polyphenolic content was analysed using the Folin-Ciocalteu method. Total flavonoid content analysis was performed using the colorimetric method.

Results: The total polyphenolic content of Z. clinopodioides is concentrated in parts of ethyl acetate (19.27%), chloroform (4.99%) and n-butanol extracts (3.94%) containing a small amount of the total polyphenolic content. The petroleum ether (0.23%) and ethanol extracts (1.64%) contain almost no polyphenolic content. The total flavonoid content of Z. clinopodioides is concentrated in parts of ethyl acetate (65.61%), chloroform (14.36%) and n-butanol extracts (10.76%) containing a small amount of the total polyphenolic content. The Z. clinopodioides Lam. ethyl acetate extract exhibits a good antioxidant activity.

Conclusion: Ethyl acetate extracts contain a large number of polyphenolic compounds (19.27%) and flavonoids (65.61%) owing to good antioxidant capacity.

Keywords: Antioxidant capacity; Ziziphora clinopodioides Lam; total flavonoids; total polyphenolic.