Matrix pre-coated MALDI MS targets for small molecule imaging in tissues

J Am Soc Mass Spectrom. 2011 Jan;22(1):192-5. doi: 10.1007/s13361-010-0013-8. Epub 2011 Jan 20.


A new sample preparation method for MALDI tissue imaging has been developed for the analysis of low molecular weight compounds that employs matrix pre-coated MALDI targets. Tissue sections need only to be transferred onto the pre-coated target before analysis for fast and easy sample preparation. Pre-coated targets have a homogenous matrix coating with uniform crystals of approximately 1-2 μm and do not require solvents that may lead to analyte delocalization within a tissue section. We report here the use of matrix pre-coated targets for imaging of lipids, peptides, and pharmaceuticals in tissues.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Female
  • Gentisates
  • Histocytochemistry / methods*
  • Lipids / analysis
  • Mice
  • Mice, Inbred C57BL
  • Microscopy, Electron, Scanning
  • Molecular Imaging / methods*
  • Peptides / analysis
  • Pharmaceutical Preparations / analysis
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*


  • Gentisates
  • Lipids
  • Peptides
  • Pharmaceutical Preparations
  • 2,5-dihydroxybenzoic acid