Influence of selenium and/or magnesium on alleviation alcohol induced oxidative stress in rats, normalization function of liver and changes in serum lipid parameters

Hum Exp Toxicol. 2011 Nov;30(11):1811-27. doi: 10.1177/0960327111401049. Epub 2011 Apr 7.


The aim of this study was to evaluate the attenuating effect of given selenium and/or magnesium on ethanol-induced oxidative stress, disturbances of liver function and cholesterol metabolism. Forty male rats were divided into five groups: C - control, Et - intoxicated with alcohol (15% solution in drinking water), Et + Mg, Et + Se, Et + Mg + Se - intoxicated with alcohol and supplemented with selenium (0.4 mg Se/l water), magnesium (100 mg Mg/l water) and combination of Se and Mg, respectively. The experiment was carried out over the 3 months. The results show that the chronic ingestion of alcohol induces lipid peroxidation and histopathological changes in liver. Supplementation with magnesium only partially alleviates oxidative stress and damages in this tissue. The both selenium alone and combination of magnesium and selenium significantly elevated total antioxidant status (TAS) in serum, activity of glutathione peroxidase and ratio of reduced glutathione to oxidized glutathione (GSH/GSSG) in liver and retarded oxidative stress and histopathological changes in this tissue. Chronic administration of ethanol (alone and with magnesium) resulted in significant decrease in the serum total cholesterol and retardation in the body weight gain in comparison with the control group. In the groups supplemented with selenium and selenium and magnesium simultaneously, concentration of total cholesterol in serum and body gains was similar to the control group. Supplementation of Se or selenium and magnesium simultaneously significantly enhances antioxidant defence and is more effective against alcohol-induced oxidative stress, disturbance of liver function and cholesterol metabolism than the separate use of magnesium.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology
  • Antioxidants / therapeutic use*
  • Dietary Supplements
  • Ethanol / toxicity*
  • Glutathione / metabolism
  • Glutathione Disulfide / metabolism
  • Glutathione Peroxidase / metabolism
  • Lipids / blood
  • Liver / drug effects
  • Liver / metabolism
  • Liver / pathology
  • Liver Diseases, Alcoholic / drug therapy*
  • Liver Diseases, Alcoholic / metabolism
  • Liver Diseases, Alcoholic / pathology
  • Magnesium / pharmacology
  • Magnesium / therapeutic use*
  • Male
  • Malondialdehyde / metabolism
  • Oxidative Stress / drug effects
  • Rats
  • Rats, Wistar
  • Selenium / pharmacology
  • Selenium / therapeutic use*
  • Superoxide Dismutase / metabolism


  • Antioxidants
  • Lipids
  • Ethanol
  • Malondialdehyde
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Glutathione
  • Selenium
  • Magnesium
  • Glutathione Disulfide