Effect of infancy-onset dietary intervention on salivary cholesterol of children: a randomized controlled trial

J Dent Res. 2011 Jul;90(7):868-73. doi: 10.1177/0022034511405328. Epub 2011 Apr 7.


This study investigated salivary cholesterol of children from 6 to 16 years of age in response to dietary intervention. One thousand sixty-two infants started in the prospective, randomized project. At 3 years of age, every fifth child was invited into the study (n=178). Of these, 148 enrolled, and 86 completed the oral sub-study at 16 years of age. The intervention aimed at restricting the child's saturated fat and cholesterol intake. Control children received no special recommendations. Every third year, paraffin-stimulated saliva samples (10.0 mL) were collected for cholesterol assays. Nutrient intakes and serum total cholesterol concentrations were regularly followed up by means of 4-day food records and blood samples. Intake of saturated fatty acids (SAFA) was lower in the intervention than in the control group (p<0.001). Salivary cholesterol concentration increased from 1.9 (±1.1) µmol/L at 6 years of age to 16.0 (±9.0) µmol/L at 16 years of age. The increase was smaller in the intervention than in the control group (p<0.001). The ratios of salivary to serum cholesterol concentrations tended to be higher in boys than in girls (p=0.07). Thus, dietary intervention was reflected in children's salivary cholesterol values more sensitively than in serum cholesterol values. (clinicaltrials.gov NCT00223600).

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Adolescent
  • Analysis of Variance
  • Body Mass Index
  • Child
  • Cholesterol / analysis*
  • Cholesterol / blood
  • Cholesterol, Dietary*
  • Diet Records
  • Diet, Fat-Restricted*
  • Fatty Acids
  • Female
  • Humans
  • Male
  • Prospective Studies
  • Saliva / chemistry*
  • Sensitivity and Specificity


  • Cholesterol, Dietary
  • Fatty Acids
  • Cholesterol

Associated data

  • ClinicalTrials.gov/NCT00223600