Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 74 (7), 920-34

Advances in Top-Down Proteomics for Disease Biomarker Discovery


Advances in Top-Down Proteomics for Disease Biomarker Discovery

David Calligaris et al. J Proteomics.


Top-down mass spectrometry strategies allow identification and characterization of proteins and protein networks by direct fragmentation. These analytical processes involve a panel of fragmentation mechanisms, some of which preserve protein post-translational modifications. Thus top-down is of special interest in clinical biochemistry to probe modified proteins as potential disease biomarkers. This review describes separating methods, mass spectrometry instrumentation, bioinformatics, and theoretical aspects of fragmentation mechanisms used for top-down analysis. The biological interest of this strategy is extensively reported regarding the characterization of post-translational modifications in biochemical pathways and the discovery of biomarkers. One has to bear in mind that quantitative aspects that are beyond the focus of this review are also of critical important for biomarker discovery. The constant evolution of technologies makes top-down strategies crucial players in clinical and basic proteomics.

Similar articles

See all similar articles

Cited by 16 PubMed Central articles

See all "Cited by" articles

MeSH terms

LinkOut - more resources