The evaluation of human papillomavirus and p53 gene mutation in benign and malignant conjunctiva and eyelid lesions

Folia Histochem Cytobiol. 2010 Dec;48(4):530-3. doi: 10.2478/v10042-010-0081-4.


Papillomas and squamous cell carcinomas are the most common conjunctival and eyelid lesions. The etiology is still unclear and recently human papillomavirus infection and p53 gene mutation have been taken into consideration. The aim of our study was the evaluation of HPV DNApresence and p53 gene mutation in 45 benign and 38 malignant squamous lesions of the conjunctiva and eyelid. For HPV detection PCR-RFLP and immunohistochemical reaction were used; for p53 gene mutation PCR-SSCP was used. Only 8.8% papillomas, 9.1% squamous cell cancers and 3.7% basal cell cancers (using PCR-RFLP method) and 26.6% papillomas, 7.4% squamous cell cancers and 9.1% basal cell cancers (using immunohisto-chemical reaction) were HPV positive. p53 gene mutation was evaluated in 24.4% papillomas, 54.5% squamous cell cancers and 22.2% basal cell cancers; most commonly in 6 and 7 exon. Human papillomavirus infection, opposite to p53 gene mutation, is not a significant etiological factor of the benign and malignant conjunctival and eyelid lesions development.

MeSH terms

  • Carcinoma, Squamous Cell / genetics
  • Carcinoma, Squamous Cell / pathology
  • Carcinoma, Squamous Cell / virology
  • Conjunctival Neoplasms / etiology*
  • Conjunctival Neoplasms / genetics
  • Conjunctival Neoplasms / virology
  • DNA, Viral / chemistry
  • Eyelid Neoplasms / etiology*
  • Eyelid Neoplasms / genetics
  • Eyelid Neoplasms / virology
  • Genes, p53*
  • Humans
  • Mutation*
  • Papillomaviridae / genetics
  • Papillomaviridae / isolation & purification*
  • Papillomavirus Infections / etiology*
  • Papillomavirus Infections / genetics
  • Papillomavirus Infections / virology
  • Polymorphism, Single-Stranded Conformational


  • DNA, Viral