Identification of a gene necessary for cell cycle arrest by a negative growth factor of yeast: FAR1 is an inhibitor of a G1 cyclin, CLN2

Cell. 1990 Nov 30;63(5):999-1011. doi: 10.1016/0092-8674(90)90503-7.


alpha factor is a negative growth factor and differentiation factor that induces G1 arrest and increases transcription of mating genes in S. cerevisiae a cells. We have identified a gene, FAR1 (for "factor arrest"), which is necessary for cell cycle arrest but not for other responses to alpha factor: far1- mutants are insensitive to arrest despite having an intact signal transduction pathway. FAR1 is a nonessential gene whose expression is induced 4- to 5-fold in a cells by alpha factor. The sequence of FAR1 indicates no significant similarities to known proteins. A null mutation in the CLN2 gene, which codes for a G1 cyclin, reverses the effect of a far1 null mutation: far1- cln2- strains arrest in response to alpha factor. We thus propose that FAR1 contributes to cell cycle arrest by inhibiting CLN2. The behavior of far1- cln2- strains indicates that products other than FAR1 are responsible for inhibiting the other G1 cyclins, CLN1 and CLN3.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cell Cycle
  • Chromosome Deletion
  • Cloning, Molecular
  • Cyclins / biosynthesis
  • Cyclins / genetics*
  • Genes, Fungal*
  • Genes, Suppressor
  • Genotype
  • Interphase
  • Mating Factor
  • Molecular Sequence Data
  • Open Reading Frames
  • Peptides / physiology
  • Pheromones
  • Recombinant Fusion Proteins / biosynthesis
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development


  • Cyclins
  • Peptides
  • Pheromones
  • Recombinant Fusion Proteins
  • Mating Factor

Associated data

  • GENBANK/M37230
  • GENBANK/M60071