Deletion of SNAP-23 results in pre-implantation embryonic lethality in mice

PLoS One. 2011 Mar 29;6(3):e18444. doi: 10.1371/journal.pone.0018444.


SNARE-mediated membrane fusion is a pivotal event for a wide-variety of biological processes. SNAP-25, a neuron-specific SNARE protein, has been well-characterized and mouse embryos lacking Snap25 are viable. However, the phenotype of mice lacking SNAP-23, the ubiquitously expressed SNAP-25 homolog, remains unknown. To reveal the importance of SNAP-23 function in mouse development, we generated Snap23-null mice by homologous recombination. We were unable to obtain newborn SNAP-23-deficient mice, and analysis of pre-implantation embryos from Snap23(Δ/wt) matings revealed that Snap23-null blastocysts were dying prior to implantation at embryonic day E3.5. Thus these data reveal a critical role for SNAP-23 during embryogenesis.

Publication types

  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Animals
  • Blastocyst / cytology
  • Blastocyst / metabolism
  • Breeding
  • Cell Death
  • Embryo Implantation*
  • Embryo Loss / metabolism*
  • Embryo Loss / pathology*
  • Female
  • Gene Deletion*
  • Gene Targeting
  • Heterozygote
  • Mice
  • Mice, Inbred C57BL
  • Pregnancy
  • Qb-SNARE Proteins / deficiency*
  • Qb-SNARE Proteins / metabolism
  • Qc-SNARE Proteins / deficiency*
  • Qc-SNARE Proteins / metabolism


  • Qb-SNARE Proteins
  • Qc-SNARE Proteins
  • Snap23 protein, mouse