MicroRNA-142-3p, a new regulator of RAC1, suppresses the migration and invasion of hepatocellular carcinoma cells

FEBS Lett. 2011 May 6;585(9):1322-30. doi: 10.1016/j.febslet.2011.03.067. Epub 2011 Apr 7.


RAC1 regulates a diverse array of cellular events including migration and invasion. MicroRNAs (miRNAs) have a key role in the regulation of gene expression. In this study, we demonstrated that microRNA-142-3p (miR-142-3p) acted as a negative regulator of human RAC1. Overexpression of miR-142-3p decreased RAC1 mRNA and protein levels. Moreover, the overexpression of miR-142-3p suppressed, while blocking of miR-142-3p increased colony formation, migration and invasion in hepatocellular carcinoma (HCC) cell lines (QGY-7703 and SMMC-7721). RAC1 overexpression without the 3'untranslated region abolished the effect of miR-142-3p in the QGY-7703 and SMMC-7721 cells. These results demonstrated that miR-142-3p directly and negatively regulates RAC1 in HCC cells, which highlights the importance of miRNAs in tumorigenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5' Untranslated Regions / genetics
  • Base Sequence
  • Blotting, Western
  • Carcinoma, Hepatocellular / genetics*
  • Carcinoma, Hepatocellular / metabolism
  • Carcinoma, Hepatocellular / pathology
  • Cell Line, Tumor
  • Cell Movement
  • Down-Regulation
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Immunohistochemistry
  • Liver / metabolism
  • Liver Neoplasms / genetics*
  • Liver Neoplasms / metabolism
  • Liver Neoplasms / pathology
  • MicroRNAs / genetics*
  • Neoplasm Invasiveness
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tumor Stem Cell Assay
  • rac1 GTP-Binding Protein / genetics*
  • rac1 GTP-Binding Protein / metabolism


  • 5' Untranslated Regions
  • MIRN142 microRNA, human
  • MicroRNAs
  • RAC1 protein, human
  • rac1 GTP-Binding Protein