Imaging surface plasmon resonance for multiplex microassay sensing of mycotoxins

Anal Bioanal Chem. 2011 Jul;400(9):3005-11. doi: 10.1007/s00216-011-4973-8. Epub 2011 Apr 12.


A prototype imaging surface plasmon resonance-based multiplex microimmunoassay for mycotoxins is described. A microarray of mycotoxin-protein conjugates was fabricated using a continuous flow microspotter device. A competitive inhibition immunoassay format was developed for the simultaneous detection of deoxynivalenol (DON) and zearalenone (ZEN), using a single sensor chip. Initial in-house validation showed limits of detection of 21 and 17 ng/mL for DON and 16 and 10 ng/mL for ZEN in extracts, which corresponds to 84 and 68 μg/kg for DON and 64 and 40 μg/kg for ZEN in maize and wheat samples, respectively. Finally, the results were critically compared with data obtained from liquid chromatography-mass spectrometry confirmatory analysis method and found to be in good agreement. The described multiplex immunoassay for the rapid screening of several mycotoxins meets European Union regulatory limits and represents a robust platform for mycotoxin analysis in food and feed samples.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Immunoassay / methods
  • Limit of Detection
  • Mycotoxins / analysis*
  • Surface Plasmon Resonance / methods*
  • Trichothecenes / analysis*
  • Triticum / microbiology
  • Zea mays / microbiology
  • Zearalenone / analysis*


  • Mycotoxins
  • Trichothecenes
  • Zearalenone
  • deoxynivalenol