Cloning and heterologous expression of Lactobacillus reuteri uroporphyrinogen III synthase/methyltransferase gene (cobA/hemD): preliminary characterization

Biotechnol Lett. 2011 Aug;33(8):1625-32. doi: 10.1007/s10529-011-0609-5. Epub 2011 Apr 12.

Abstract

To clone, express and characterize uroporphyrinogen III synthase/methyltransferase gene (cobA/hemD) from Lactobacillus reuteri. Some strains of Lb. reuteri produce cobalamin (vitamin B(12)). Cobalamin biosynthesis relies on the sequential action of more than 25 enzymes in a complex metabolic pathway. We have cloned, expressed and characterized the gene in Lb. reuteri that codes for the S-adenosy L: -methionine uroprophyrinogen III methyltransferase/synthase (CobA/HemD), a key bifunctional enzyme in the biosynthesis of cobalamin and other tetrapyrrols.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / biosynthesis
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics
  • Base Sequence
  • Cloning, Molecular
  • Computer Simulation
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Limosilactobacillus reuteri / enzymology*
  • Limosilactobacillus reuteri / genetics
  • Methyltransferases / biosynthesis*
  • Methyltransferases / chemistry
  • Methyltransferases / genetics*
  • Molecular Sequence Data
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Spectrometry, Fluorescence
  • Uroporphyrinogen III Synthetase / biosynthesis*
  • Uroporphyrinogen III Synthetase / chemistry
  • Uroporphyrinogen III Synthetase / genetics*

Substances

  • Bacterial Proteins
  • Recombinant Proteins
  • Methyltransferases
  • uroporphyrin-III C-methyltransferase
  • Uroporphyrinogen III Synthetase