The stability of an external ring fixator mainly depends on wire tension. Wire fixators should maintain the tension during both wire clamping to the ring and removal of the tensioner device. In the present study the loss in wire tension related to fixator clamping and wire tensioner removal using three different wire fixator designs was studied. The fixators were based on two different cannulated bolts and a washer. Effects from two different pretension levels in combination with three different bolt torque levels upon loss in wire tension were described. Emitted wire vibration frequency was used to assess the corresponding wire tension. Wire tension was determined after each wire fixator tightening and after the removal of the wire tensioner. Increased bolt torque led to a small decrease in tension for both pretension levels. A considerable higher tension loss was measured when removing the wire tensioner. In all cases, the combination of a new cannulated bolt and a washer maintained the highest tension.