Production of specific anti-rat 5-HT1A receptor antibodies in rabbits injected with a synthetic peptide

Neurosci Lett. 1990 Oct 16;118(2):189-92. doi: 10.1016/0304-3940(90)90623-h.


Polyclonal antibodies were raised by the repeated injection of rabbits with a synthetic peptide corresponding to a highly selective portion (amino acid residues 243 to 268) of the amino acid sequence of the rat 5-HT1A receptor. The anti-peptide antiserum allowed the immunoprecipitation of 5-HT1A receptors but not of other 5-HT1 sites solubilized from rat hippocampal membranes. Immunoautoradiographic labelling of rat brain sections with the anti-peptide antiserum was superimposed with the autoradiographic distribution of 5-HT1A sites labelled by the selective radioligand [3H]8-OH-DPAT.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 8-Hydroxy-2-(di-n-propylamino)tetralin
  • Amino Acid Sequence
  • Animals
  • Antibody Formation*
  • Antibody Specificity
  • Autoradiography
  • Injections
  • Male
  • Molecular Sequence Data
  • Peptide Fragments / immunology*
  • Precipitin Tests
  • Rabbits / immunology
  • Rats / immunology
  • Rats, Inbred Strains
  • Receptors, Serotonin / immunology*
  • Tetrahydronaphthalenes / metabolism
  • Tritium


  • Peptide Fragments
  • Receptors, Serotonin
  • Tetrahydronaphthalenes
  • Tritium
  • 8-Hydroxy-2-(di-n-propylamino)tetralin