Immobilization of human carbonic anhydrase on gold nanoparticles assembled onto amine/thiol-functionalized mesoporous SBA-15 for biomimetic sequestration of CO2

Langmuir. 2011 May 17;27(10):6227-34. doi: 10.1021/la105029h. Epub 2011 Apr 13.

Abstract

A biocatalyst was synthesized by immobilizing human carbonic anhydrase onto gold nanoparticles assembled over amine/thiol-functionalized mesoporous SBA-15. The physicochemical properties of the functionalized mesoporous SBA-15 were obtained by XRD, BET, FE SEM, HR TEM, EDS, and zeta potential analysis. The biocatalytic performance was studied for para-nitrophenyl acetate (p-NPA) hydrolysis. The kinetic parameters K(m) were found to be 22.35 and 27.75 mM, and K(cat)/K(m) values were 1514.09 and 1612.25 M(-1) s(-1) for HCA immobilized on gold nanoparticles assembled on amine/thiol-functionalized mesoporous SBA-15 (HCA/Au/APTES/SBA-15 and HCA/Au/MPTES/SBA-15), respectively. These HCA/Au/APTES/SBA-15 and HCA/Au/MPTES/SBA-15 were investigated for biocatalytic hydration of CO(2) and its precipitation as CaCO(3). The amount of CaCO(3) precipitated over HCA/Au/MPTES/SBA-15 was nearly the same as that precipitated over free HCA. Storage stability and reusability studies suggested that HCA/Au/MPTES/SBA-15 retained its activity even after 20 days storage at 25 °C and 20 recycling runs. The present results demonstrate that HCA/Au/MPTES/SBA-15 and HCA/Au/APTES/SBA-15 are highly efficient potential nanobiocatalysts for industrial-scale CO(2) sequestration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amines / chemistry
  • Biocatalysis
  • Biomimetics / methods*
  • Carbon Dioxide / chemistry
  • Carbon Dioxide / isolation & purification*
  • Carbon Dioxide / metabolism
  • Carbonic Anhydrases / chemistry*
  • Carbonic Anhydrases / metabolism
  • Enzymes, Immobilized / chemistry*
  • Enzymes, Immobilized / metabolism
  • Gold / chemistry*
  • Humans
  • Hydrolysis
  • Metal Nanoparticles / chemistry*
  • Phenylacetates / chemistry
  • Porosity
  • Propylamines
  • Silanes / chemistry
  • Silicon Dioxide / chemistry*
  • Sulfhydryl Compounds / chemistry

Substances

  • Amines
  • Enzymes, Immobilized
  • Phenylacetates
  • Propylamines
  • SBA-15
  • Silanes
  • Sulfhydryl Compounds
  • Carbon Dioxide
  • Gold
  • Silicon Dioxide
  • Carbonic Anhydrases
  • phenylacetic acid
  • amino-propyl-triethoxysilane