Molecular analysis of common wheat genes encoding three types of cytosolic heat shock protein 90 (Hsp90): functional involvement of cytosolic Hsp90s in the control of wheat seedling growth and disease resistance

New Phytol. 2011 Jul;191(2):418-31. doi: 10.1111/j.1469-8137.2011.03715.x. Epub 2011 Apr 13.

Abstract

Heat shock protein 90 (Hsp90) molecular chaperones play important roles in plant growth and responses to environmental stimuli. However, little is known about the genes encoding Hsp90s in common wheat. Here, we report genetic and functional analysis of the genes specifying cytosolic Hsp90s in this species. Three groups of homoeologous genes (TaHsp90.1, TaHsp90.2 and TaHsp90.3), encoding three types of cytosolic Hsp90, were isolated. The loci containing TaHsp90.1, TaHsp90.2 and TaHsp90.3 genes were assigned to groups 2, 7 and 5 chromosomes, respectively. TaHsp90.1 genes exhibited higher transcript levels in the stamen than in the leaf, root and culm. TaHsp90.2 and TaHsp90.3 genes were more ubiquitously transcribed in the vegetative and reproductive organs examined. Decreasing the expression of TaHsp90.1 genes through virus-induced gene silencing (VIGS) caused pronounced inhibition of wheat seedling growth, whereas the suppression of TaHsp90.2 or TaHsp90.3 genes via VIGS compromised the hypersensitive resistance response of the wheat variety Suwon 11 to stripe rust fungus. Our work represents the first systematic determination of wheat genes encoding cytosolic Hsp90s, and provides useful evidence for the functional involvement of cytosolic Hsp90s in the control of seedling growth and disease resistance in common wheat.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Basidiomycota / physiology*
  • Chromosome Mapping
  • Chromosomes, Plant / genetics
  • Cytosol / chemistry
  • DNA, Complementary
  • Exons / genetics
  • Gene Expression Regulation, Plant
  • Gene Knockdown Techniques
  • Gene Silencing
  • HSP90 Heat-Shock Proteins / genetics
  • HSP90 Heat-Shock Proteins / metabolism*
  • Introns / genetics
  • Organ Specificity / genetics
  • Phenotype
  • Phylogeny
  • Plant Diseases / immunology*
  • Plant Diseases / microbiology
  • Plant Immunity / immunology*
  • Plant Leaves / immunology
  • Plant Leaves / microbiology
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Seedlings / growth & development
  • Seedlings / immunology
  • Seedlings / microbiology
  • Triticum / genetics
  • Triticum / immunology
  • Triticum / microbiology
  • Triticum / physiology*

Substances

  • DNA, Complementary
  • HSP90 Heat-Shock Proteins
  • Plant Proteins