Functionalized micromachines for selective and rapid isolation of nucleic acid targets from complex samples

Nano Lett. 2011 May 11;11(5):2083-7. doi: 10.1021/nl2005687. Epub 2011 Apr 14.

Abstract

The transport properties of single-strand DNA probe-modified self-propelling micromachines are exploited for "on-the-fly" hybridization and selective single-step isolation of target nucleic acids from "raw" microliter biological samples (serum, urine, crude E. coli lysate, saliva). The rapid movement of the guided modified microrockets induces fluid convection, which enhances the hybridization efficiency, thus enabling the rapid and selective isolation of nucleic acid targets from untreated samples. The integration of these autonomous microrockets into a lab-on-chip device that provides both nucleic acid isolation and downstream analysis could thus be attractive for diverse applications.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biosensing Techniques
  • DNA / chemistry*
  • DNA, Complementary / metabolism
  • Drug Delivery Systems
  • Escherichia coli / metabolism
  • Humans
  • Ions
  • Microscopy, Fluorescence / methods
  • Motion
  • Nucleic Acid Hybridization
  • Nucleic Acids / chemistry*
  • Oligonucleotide Array Sequence Analysis / methods*
  • Serum / metabolism
  • Time Factors
  • Urine

Substances

  • DNA, Complementary
  • Ions
  • Nucleic Acids
  • DNA