Prognostic importance of Claudin-1 and Claudin-4 expression in colon carcinomas

Pathol Res Pract. 2011 May 15;207(5):285-9. doi: 10.1016/j.prp.2011.01.011. Epub 2011 Apr 13.


In this study, we analyzed Claudin-1 and Claudin-4 expressions in colon carcinomas. We investigated the relationship between the expression of these tight junction proteins and clinicopathologic parameters. Claudin-1 and 4 expressions were determined by immunohistochemical methods, and the rate of cells expressing these tight junction proteins were calculated with stereologic methods. Fifty-nine colon cancer cases were enrolled in the study group. Claudin-1 and 4 expressions were found to be significantly lower in cases with lymph node metastasis. Mean staining rates of Claudin-1 and 4 in lymph node (+) cases were 36.1±20.1 and 58±28.9, while in lymph node (-) cases, these were 63.8±25.9 and 72.3±25.6, respectively (p=0.0005 for Claudin-1, p=0.049 for Claudin-4). The mean staining rate for Claudin-1 in adenomatous polyps was significantly higher than incarcinomas (77.13±23.4 and 50.6±26.93, respectively) (p=0.003), while it was quite similar for Claudin-4 (65.4±26.9 and 65.3±27.9, respectively). In this study, we demonstrated Claudin-1 and 4 expressions in colon cancer cases. Claudin-1 expression seems to be more prominent in adenomatous polyps as compared with cancer cases. Expression of Claudin-1 decreases significantly in the presence of lymph node metastasis and diminishes in mucinous carcinoma cases, indicating a negative correlation between Claudin-1 expression and neoplastic progression.

MeSH terms

  • Adenocarcinoma, Mucinous / diagnosis
  • Adenocarcinoma, Mucinous / metabolism*
  • Adenocarcinoma, Mucinous / secondary
  • Adenomatous Polyps / diagnosis
  • Adenomatous Polyps / metabolism*
  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor / metabolism
  • Claudin-1
  • Claudin-4
  • Colonic Neoplasms / diagnosis
  • Colonic Neoplasms / metabolism*
  • Female
  • Humans
  • Immunohistochemistry / methods
  • Lymph Nodes / metabolism
  • Lymph Nodes / pathology
  • Lymphatic Metastasis
  • Male
  • Membrane Proteins / metabolism*
  • Middle Aged
  • Prognosis
  • Young Adult


  • Biomarkers, Tumor
  • CLDN1 protein, human
  • CLDN4 protein, human
  • Claudin-1
  • Claudin-4
  • Membrane Proteins