Dietary selenium affects host selenoproteome expression by influencing the gut microbiota

FASEB J. 2011 Jul;25(7):2492-9. doi: 10.1096/fj.11-181990. Epub 2011 Apr 14.

Abstract

Colonization of the gastrointestinal tract and composition of the microbiota may be influenced by components of the diet, including trace elements. To understand how selenium regulates the intestinal microflora, we used high-throughput sequencing to examine the composition of gut microbiota of mice maintained on selenium-deficient, selenium-sufficient, and selenium-enriched diets. The microbiota diversity increased as a result of selenium in the diet. Specific phylotypes showed differential effects of selenium, even within a genus, implying that selenium had unique effects across microbial taxa. Conventionalized germ-free mice subjected to selenium diets gave similar results and showed an increased diversity of the bacterial population in animals fed with higher levels of selenium. Germ-free mice fed selenium diets modified their selenoproteome expression similar to control mice but showed higher levels and activity of glutathione peroxidase 1 and methionine-R-sulfoxide reductase 1 in the liver, suggesting partial sequestration of selenium by the gut microorganisms, limiting its availability for the host. These changes in the selenium status were independent of the levels of other trace elements. The data show that dietary selenium affects both composition of the intestinal microflora and colonization of the gastrointestinal tract, which, in turn, influence the host selenium status and selenoproteome expression.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural

MeSH terms

  • Animals
  • Blotting, Western
  • Dietary Supplements
  • Feces / microbiology
  • Gastrointestinal Tract / drug effects*
  • Gastrointestinal Tract / metabolism
  • Gastrointestinal Tract / microbiology
  • Gene Expression / drug effects*
  • Germ-Free Life
  • Glutathione Peroxidase / metabolism
  • Intestinal Mucosa / metabolism
  • Intestines / drug effects
  • Intestines / microbiology
  • Male
  • Metagenome / genetics
  • Methionine Sulfoxide Reductases / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Proteome / genetics*
  • Proteome / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Selenium / administration & dosage
  • Selenium / pharmacology*
  • Selenoproteins / blood
  • Selenoproteins / genetics*
  • Selenoproteins / metabolism
  • Sequence Analysis, DNA
  • Trace Elements / metabolism

Substances

  • Proteome
  • Selenoproteins
  • Trace Elements
  • glutathione peroxidase GPX1
  • Glutathione Peroxidase
  • Methionine Sulfoxide Reductases
  • methionine sulfoxide reductase
  • Selenium