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. 2011 Feb;11(1):42-9.
doi: 10.4110/in.2011.11.1.42. Epub 2011 Feb 28.

Anti-inflammatory and Anti-oxidative Effects of Korean Red Ginseng Extract in Human Keratinocytes

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Free PMC article

Anti-inflammatory and Anti-oxidative Effects of Korean Red Ginseng Extract in Human Keratinocytes

Chang-Eui Hong et al. Immune Netw. .
Free PMC article

Abstract

Background: In this study, we have investigated the effect of Korean red ginseng (KRG) extracts on the production of TNF-α and IL-8 in human keratinocytes. Also, to examine the antioxidative effect of red ginseng extracts, free radical scavenging activity and superoxide dismutase (SOD) activity in human dermal fibroblasts was measured.

Methods: To investigate the effect of KRG in atopic dermatitis, we measured the level of TNF-α and IL-8 secretion in LPS-stimulated human keratinocytes after the treatment of KRG extracts using enzyme-linked immunosorbent assay. Anti-oxidative activity was investigated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and SOD activity.

Results: The stimulation of human keratinocytes with KRG extracts shifted the LPS-induced cytokine secretion toward a more immunosuppressive response. KRG dose-dependently decreased TNF-α and IL-8 production in HaCaT cells and a significant inhibition of TNF-α was shown when cells were treated with 500 and 1,000 µg/ml of KRG extracts. Additionally, KRG extracts showed DPPH radical scavenging and SOD activity in a dose-dependent manner. Particularly, SOD activities of concentrations higher than 60 µg/ml of KRG extracts were significantly different in human dermal fibroblast cells.

Conclusion: Based on this study, KRG extracts may be a useful immunosuppressive agent in the treatment of atopic dermatitis.

Keywords: Anti-oxidation; Atopic dermatitis; IL-8; Korean red ginseng; TNF-α.

Conflict of interest statement

The authors have no financial conflict of interest.

Figures

Figure 1
Figure 1
Cell viability of human keratinocyte cells (HaCaT) when treated with Korean red ginseng (KRG) extracts. HaCaT cells were treated with various concentrations (100, 200, 400, 800, 1,000, 2,000, 4,000, 6,000, 8,000, and 10,000 µg/ml) of KRG extract for 48 h and the cytotoxicity was measured by MTT assay colorimetric dye reduction method.
Figure 2
Figure 2
Cell viability of human dermal fibroblasts (HDF-N) when treated with Korean red ginseng (KRG) extracts. HDF-N cells were treated with various concentrations (100, 200, 400, 800, 1,000, 2,000, 4,000, 6,000, 8,000, and 10,000 µg/ml) of KRG extract for 48 h and the cytotoxicity was measured by MTT assay colorimetric dye reduction method.
Figure 3
Figure 3
Inhibition of TNF-α secretion in HaCaT cells by Korean red ginseng (KRG) extracts. Cells were stimulated with 1 µg/ml LPS and was treated with various concentrations of KRG extracts for 48 h. Significant difference in comparison with control at *p<0.05 and **p<0.01.
Figure 4
Figure 4
Inhibition of IL-8 secretion in HaCaT cells by Korean red ginseng (KRG) extracts. Cells were stimulated with 1 µg/ml LPS and was treated with various concentrations of KRG extracts for 48 h. *Significant difference in comparison with control at p<0.05.

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