Death-associated Protein Kinase 1 Phosphorylates Pin1 and Inhibits Its Prolyl Isomerase Activity and Cellular Function

Mol Cell. 2011 Apr 22;42(2):147-59. doi: 10.1016/j.molcel.2011.03.005. Epub 2011 Apr 14.

Abstract

Pin1 is a phospho-specific prolyl isomerase that regulates numerous key signaling molecules and whose deregulation contributes to disease notably cancer. However, since prolyl isomerases are often believed to be constitutively active, little is known whether and how Pin1 catalytic activity is regulated. Here, we identify death-associated protein kinase 1 (DAPK1), a known tumor suppressor, as a kinase responsible for phosphorylation of Pin1 on Ser71 in the catalytic active site. Such phosphorylation fully inactivates Pin1 catalytic activity and inhibits its nuclear location. Moreover, DAPK1 inhibits the ability of Pin1 to induce centrosome amplification and cell transformation. Finally, Pin1 pSer71 levels are positively correlated with DAPK1 levels and negatively with centrosome amplification in human breast cancer. Thus, phosphorylation of Pin1 Ser71 by DAPK1 inhibits its catalytic activity and cellular function, providing strong evidence for an essential role of the Pin1 enzymatic activity for its cellular function.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Active Transport, Cell Nucleus
  • Animals
  • Apoptosis Regulatory Proteins / genetics
  • Apoptosis Regulatory Proteins / metabolism*
  • Breast Neoplasms / enzymology*
  • Calcium-Calmodulin-Dependent Protein Kinases / genetics
  • Calcium-Calmodulin-Dependent Protein Kinases / metabolism*
  • Catalytic Domain
  • Cell Cycle
  • Cell Nucleus / enzymology
  • Cell Proliferation
  • Cell Transformation, Neoplastic / metabolism
  • Centrosome / metabolism
  • Death-Associated Protein Kinases
  • Enzyme Stability
  • Female
  • HeLa Cells
  • Humans
  • Immunohistochemistry
  • Mice
  • Mice, Knockout
  • Microscopy, Fluorescence
  • Mutation
  • NIH 3T3 Cells
  • NIMA-Interacting Peptidylprolyl Isomerase
  • Peptidylprolyl Isomerase / antagonists & inhibitors
  • Peptidylprolyl Isomerase / genetics
  • Peptidylprolyl Isomerase / metabolism*
  • Phosphorylation
  • Protein Interaction Domains and Motifs
  • Protein Interaction Mapping
  • Recombinant Fusion Proteins / metabolism
  • Serine
  • Signal Transduction*
  • Time Factors
  • Tissue Array Analysis
  • Transfection

Substances

  • Apoptosis Regulatory Proteins
  • NIMA-Interacting Peptidylprolyl Isomerase
  • Recombinant Fusion Proteins
  • Serine
  • DAPK1 protein, human
  • Dapk1 protein, mouse
  • Death-Associated Protein Kinases
  • Calcium-Calmodulin-Dependent Protein Kinases
  • PIN1 protein, human
  • Peptidylprolyl Isomerase
  • Pin1 protein, mouse