Embryonic ectoderm development protein is regulated by microRNAs in human neural tube defects

Am J Obstet Gynecol. 2011 Jun;204(6):544.e9-17. doi: 10.1016/j.ajog.2011.01.045. Epub 2011 Apr 16.


Objective: The objective of the study was to investigate the expression and regulation of polycomb group (PcG) proteins in human neural tube defects (NTDs).

Study design: PcG proteins in human NTD fetuses and age-matched controls were detected by Western blot. The relation between PcG proteins and microribonucleic acids was predicted and confirmed by the bioinformatics method, real-time polymerase chain reaction (PCR), dual-luciferase activity assay, and Western blot. The trimethyl condition of histone H3 Lys27 (H3K27) was detected by immunohistochemical and immunofluorescence.

Results: Embryonic ectoderm development protein (EED) was differentially detected in placenta, cerebral cortex, and spinal cord from NTDs and age-matched controls. MiR-30b can interact with 3'-untranslated region (UTR) of Eed and regulate endogenous EED expression in neural tissues. In addition, we found an inverse relationship between the miR-30b expression and the amount of trimethyl H3K27.

Conclusion: Differential expression of EED exists in the nerves system in human NTDs and that is regulated by miR-30b.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Female
  • Fetus
  • Gene Expression Regulation
  • Humans
  • Male
  • MicroRNAs / physiology*
  • Neural Tube Defects / genetics*
  • Polycomb Repressive Complex 2
  • Repressor Proteins / genetics*


  • EED protein, human
  • MicroRNAs
  • Repressor Proteins
  • Polycomb Repressive Complex 2