Co-localization of prothrombin fragment F1+2 and VEGF-R2-bound VEGF in human colon cancer

Anticancer Res. 2011 Mar;31(3):843-7.


Background: Colon cancer (CC) is frequently complicated by thromboembolic episodes. Thrombin plays a role in angiogenesis and among others induces the synthesis of vascular endothelial growth factor (VEGF) and its receptors (VEGFR-1 and VEGFR-2). The aim of this study was to assess the expression of prothrombin fragment F1+2 (F1+2), a byproduct in thrombin generation (indicating the presence of thrombin), in relation to the presence of VEGFR-2-bound VEGF (VEGF:VEGFR-2), as an indicator of VEGFR-2 activation in human CC tissue.

Materials and methods: Immunohistochemical ABC and double staining studies were performed using antibodies against F1+2 and VEGF:VEGFR-2 in 59 specimens obtained from CC patients.

Results: Medium and high expression of both F1+2 and VEGF:VEGF2 in association with CC cells and endothelial cells was demonstrated. Moreover, coexpression of F1+2 and VEGF:VEGFR-2 was observed in the cells.

Conclusion: The results may suggest a possible functional interaction between thrombin and VEGF-R2 stimulation in human CC in vivo.

MeSH terms

  • Adult
  • Colonic Neoplasms / metabolism*
  • Colonic Neoplasms / pathology
  • Female
  • Humans
  • Immunohistochemistry
  • Male
  • Middle Aged
  • Peptide Fragments / metabolism*
  • Protein Transport
  • Prothrombin / metabolism*
  • Vascular Endothelial Growth Factor A / metabolism*
  • Vascular Endothelial Growth Factor Receptor-2 / metabolism*


  • Peptide Fragments
  • VEGFA protein, human
  • Vascular Endothelial Growth Factor A
  • prothrombin fragment 1.2
  • Prothrombin
  • Vascular Endothelial Growth Factor Receptor-2