Enamel and dentine remineralization by nano-hydroxyapatite toothpastes

J Dent. 2011 Jun;39(6):430-7. doi: 10.1016/j.jdent.2011.03.008. Epub 2011 Apr 8.

Abstract

Objectives: This in vitro study evaluated the effects of nano-hydroxyapatite (n-HAp) toothpastes on remineralization of bovine enamel and dentine subsurface lesions.

Methods: Specimens were demineralized, randomly divided into five groups, and exposed to an aqueous remineralizing solution for two and five weeks (37 °C). Brushing procedures were performed with the respective toothpaste/storage solution slurry twice daily (2 × 5 s; total contact time of the slurries 2 × 120s/d): storage in remineralizing solution only (0); additional brushing with B (20 wt% zinc carbonate nano-hydroxyapatite, ZnCO(3)/n-HAp); BS (24 wt% ZnCO(3)/n-HAp); E (0.14 wt% amine fluoride); or A (7 wt% pure n-HAp). Differences in mineral loss (ΔΔZ) before and after storage/treatment were microradiographically evaluated.

Results: Dentine groups 0, B, BS, and A showed significantly higher ΔΔZ values compared to E (p < 0.05; ANOVA). Enamel ΔΔZ values of group A were significantly higher compared to group E (p < 0.05), whilst no significant differences of these groups could be observed compared to 0, B, and BS (p > 0.05).

Conclusions: With the in vitro conditions chosen, toothpastes containing n-HAp revealed higher remineralizing effects compared to amine fluoride toothpastes with bovine dentine, and comparable trends were obtained for enamel.

Publication types

  • Comparative Study

MeSH terms

  • Amines / therapeutic use
  • Animals
  • Calcium Phosphates / therapeutic use
  • Carbonates / therapeutic use
  • Cariostatic Agents / therapeutic use*
  • Cattle
  • Dental Enamel / drug effects*
  • Dental Enamel / pathology
  • Dentin / drug effects*
  • Dentin / pathology
  • Durapatite / therapeutic use*
  • Fluorides / therapeutic use
  • Microradiography
  • Nanostructures / therapeutic use*
  • Random Allocation
  • Temperature
  • Time Factors
  • Tooth Demineralization / pathology
  • Tooth Demineralization / prevention & control
  • Tooth Remineralization / methods*
  • Toothbrushing / methods
  • Toothpastes / therapeutic use*
  • Zinc Compounds / therapeutic use

Substances

  • Amines
  • Calcium Phosphates
  • Carbonates
  • Cariostatic Agents
  • Toothpastes
  • Zinc Compounds
  • Durapatite
  • calcium phosphate
  • zinc carbonate
  • Fluorides