Rosmarinic acid attenuates 2,4-dinitrofluorobenzene-induced atopic dermatitis in NC/Nga mice

Int Immunopharmacol. 2011 Sep;11(9):1271-7. doi: 10.1016/j.intimp.2011.04.007. Epub 2011 Apr 17.

Abstract

Atopic dermatitis (AD) is one of the most common skin diseases, and its incidence is increasing in industrialized countries. Furthermore, the epicutaneous application of a hapten, such as 2,4-dinitrofluorobenzene (DNFB), evokes an AD-like lesion in NC/Nga mice under specific pathogen-free (SPF) conditions. Rosmarinic acid (RA) is a secondary metabolite that is frequently found in herbs, and has anti-inflammatory, anti-oxidant, and anti-microbial effects. In this study, we studied whether RA is an effective treatment against DNFB-induced AD-like skin lesions in NC/Nga mice. RA at 1 or 5 μM was found to suppress the productions of interferon (IFN)-γ and interleukin (IL)-4 significantly by activated CD4(+) T cells. Furthermore, an intraperitoneal injection of RA at 10 or 50 mg/kg significantly inhibited skin lesion development and ear thickness and total serum IgE level increases in DNFB-treated NC/Nga mice. In addition, intraperitoneal administered RA at 10 or 50 mg/kg significantly inhibited the infiltrations of CD4(+) T, CD8(+) T, and mast cells into DNFB-induced skin lesions in NC/Nga mice. This study suggests that RA suppresses the development of AD-like dermatitis in DNFB-treated NC/Nga mice by reducing IFN-γ and IL-4 production by activated T cells and total serum IgE levels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD4-Positive T-Lymphocytes / drug effects
  • CD4-Positive T-Lymphocytes / immunology
  • CD4-Positive T-Lymphocytes / metabolism
  • CD8-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / metabolism
  • Cinnamates / pharmacology*
  • Depsides / pharmacology*
  • Dermatitis, Atopic / chemically induced
  • Dermatitis, Atopic / drug therapy*
  • Dermatitis, Atopic / immunology*
  • Dermatitis, Atopic / pathology
  • Dinitrofluorobenzene / antagonists & inhibitors*
  • Dinitrofluorobenzene / pharmacology
  • Haptens / pharmacology
  • Immunoglobulin E / blood
  • Immunoglobulin E / immunology
  • Interferon-gamma / antagonists & inhibitors
  • Interferon-gamma / metabolism
  • Interleukin-4 / antagonists & inhibitors
  • Interleukin-4 / immunology
  • Interleukin-4 / metabolism
  • Lymphocyte Activation / drug effects
  • Lymphocyte Activation / immunology
  • Male
  • Mast Cells / immunology
  • Mast Cells / metabolism
  • Mast Cells / pathology
  • Mice
  • Skin / drug effects*
  • Skin / immunology*
  • Skin / pathology
  • Skin Diseases / immunology
  • Skin Diseases / metabolism
  • Skin Diseases / pathology

Substances

  • Cinnamates
  • Depsides
  • Haptens
  • Interleukin-4
  • Immunoglobulin E
  • Interferon-gamma
  • Dinitrofluorobenzene
  • rosmarinic acid