Mechanical manipulation of neurons to control axonal development

J Vis Exp. 2011 Apr 10;(50):2509. doi: 10.3791/2509.

Abstract

Cell manipulations and extension of neuronal axons can be accomplished with calibrated glass micro-fibers capable of measuring and applying forces in the 10-1000 μdyne range. Force measurements are obtained through observation of the Hookean bending of the glass needles, which are calibrated by a direct and empirical method. Equipment requirements and procedures for fabricating, calibrating, treating, and using the needles on cells are fully described. The force regimes previously used and different cell types to which these techniques have been applied demonstrate the flexibility of the methodology and are given as examples for future investigation. The technical advantages are the continuous 'visualization' of the forces produced by the manipulations and the ability to directly intervene in a variety of cellular events. These include direct stimulation and regulation of axonal growth and retraction; as well as detachment and mechanical measurements on any type of cultured cell.

Publication types

  • Video-Audio Media

MeSH terms

  • Animals
  • Axons / physiology*
  • Biomechanical Phenomena
  • Chickens
  • Cytological Techniques / instrumentation
  • Cytological Techniques / methods*
  • Glass
  • Needles
  • Neurons / physiology*