Analytical strategies for assessment of human metabolites in preclinical safety testing

Anal Chem. 2011 Jul 1;83(13):5028-36. doi: 10.1021/ac200349g. Epub 2011 Apr 20.


Strategies for the detection, identification, and quantification of in vivo drug metabolites from non-radiolabeled studies are reviewed with an emphasis on the utilization of accurate-mass-based data mining tools. New approaches to the determination of coverage of human drug metabolites in preclinical species without using radiolabeled drugs or synthetic standards are also discussed.

MeSH terms

  • Biotransformation
  • Chromatography, Liquid
  • Humans
  • Mass Spectrometry
  • Toxicity Tests*