Direct assessments of the antioxidant effects of the novel collagen peptide on reactive oxygen species using electron spin resonance spectroscopy

J Pharmacol Sci. 2011;116(1):97-106. doi: 10.1254/jphs.10321fp. Epub 2011 Apr 21.

Abstract

In the present study, we evaluated the antioxidant effects of a pepsin-treated novel collagen peptide (P-NCP) on reactive oxygen species (ROS) such as hydroxyl radical (HO(•)), superoxide anion radical (O(2)(•-)), and singlet oxygen ((1)O(2)), and the effects on cell viability after ultraviolet ray (UV) irradiation of human fibroblasts. We confirmed, using electron spin resonance, that P-NCP directly inhibited HO(•) and (1)O(2). Furthermore, addition of P-NCP to fibroblasts inhibited cell death induced by UVA (400-315 nm) irradiation in a dose-dependent manner. In addition, the antioxidant effect on (1)O(2) was observed in the peptide fractions rich in Gly, Pro, Hyp, Glu, Ala, and Arg. We found that Gly, Hyp, Glu, and Ala directly scavenged (1)O(2). These results indicated that a peptide sequence including Gly, Hyp, Glu, and Ala could play a key role in the antioxidant effects of P-NCP on (1)O(2). It was suggested that P-NCP can inhibit photo-aging related to ROS owing to its antioxidant effects.

MeSH terms

  • Amino Acids / analysis
  • Amino Acids / chemistry
  • Animals
  • Antioxidants / chemistry*
  • Antioxidants / isolation & purification
  • Antioxidants / metabolism
  • Antioxidants / pharmacology
  • Cattle
  • Cell Line
  • Cell Survival / drug effects
  • Chromatography, Gel
  • Collagen Type I / metabolism*
  • Electron Spin Resonance Spectroscopy
  • Free Radical Scavengers / chemistry
  • Free Radical Scavengers / isolation & purification
  • Free Radical Scavengers / metabolism
  • Free Radical Scavengers / pharmacology
  • Humans
  • Hydrolysis
  • Male
  • Peptide Fragments / chemistry*
  • Peptide Fragments / isolation & purification
  • Peptide Fragments / metabolism
  • Peptide Fragments / pharmacology
  • Protein Hydrolysates / chemistry*
  • Protein Hydrolysates / metabolism
  • Protein Hydrolysates / pharmacology
  • Reactive Oxygen Species / antagonists & inhibitors
  • Singlet Oxygen / antagonists & inhibitors
  • Skin / drug effects
  • Skin / radiation effects
  • Skin Aging / drug effects
  • Ultraviolet Rays / adverse effects

Substances

  • Amino Acids
  • Antioxidants
  • Collagen Type I
  • Free Radical Scavengers
  • Peptide Fragments
  • Protein Hydrolysates
  • Reactive Oxygen Species
  • Singlet Oxygen