Hydrogen-rich saline is cerebroprotective in a rat model of deep hypothermic circulatory arrest

Neurochem Res. 2011 Aug;36(8):1501-11. doi: 10.1007/s11064-011-0476-4. Epub 2011 Apr 22.


Deep hypothermic circulatory arrest (DHCA) has been widely used in the operations involving the aortic arch and brain aneurysm since 1950s; but prolonged DHCA contributes significantly to neurological deficit which remains a major cause of postoperative morbidity and mortality. It has been reported that hydrogen exerts a therapeutic antioxidant activity by selectively reducing hydroxyl radical. In this study, DHCA treated rats developed a significant oxidative stress, inflammatory reaction and apoptosis. The administration of HRS resulted in a significant decrease in the brain injury, together with lower production of IL-1β, TNF-α, 8-OHdG and MDA as well as decreased activity of NOS while increased activity of SOD. The apoptotic index as well as the expressions of caspase-3 in brain tissue was significantly decreased after treatment. HRS administration significantly attenuated the severity of DHCA induced brain injury by mechanisms involving amelioration of oxidative stress, down-regulation of inflammatory factors and reduction of apoptosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / drug effects
  • Brain Injuries / pathology
  • Brain Injuries / prevention & control*
  • Caspase 3 / metabolism
  • Circulatory Arrest, Deep Hypothermia Induced / adverse effects*
  • Circulatory Arrest, Deep Hypothermia Induced / mortality
  • Hydrogen / pharmacology
  • Hydrogen / therapeutic use*
  • Inflammation / prevention & control
  • Male
  • Neuroprotective Agents / pharmacology
  • Neuroprotective Agents / therapeutic use*
  • Oxidative Stress / drug effects
  • Random Allocation
  • Rats
  • Rats, Sprague-Dawley
  • Sodium Chloride / chemistry*
  • Sodium Chloride / pharmacology
  • Sodium Chloride / therapeutic use*


  • Neuroprotective Agents
  • Sodium Chloride
  • Hydrogen
  • Caspase 3