Next-generation sequencing to generate interactome datasets

Nat Methods. 2011 Jun;8(6):478-80. doi: 10.1038/nmeth.1597. Epub 2011 Apr 24.


Next-generation sequencing has not been applied to protein-protein interactome network mapping so far because the association between the members of each interacting pair would not be maintained in en masse sequencing. We describe a massively parallel interactome-mapping pipeline, Stitch-seq, that combines PCR stitching with next-generation sequencing and used it to generate a new human interactome dataset. Stitch-seq is applicable to various interaction assays and should help expand interactome network mapping.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Databases, Protein / statistics & numerical data*
  • Humans
  • Open Reading Frames
  • Polymerase Chain Reaction
  • Protein Interaction Mapping / statistics & numerical data*
  • Sequence Analysis, DNA / statistics & numerical data*
  • Two-Hybrid System Techniques